Molecular Probes Alexa 488

The secondary antibodies used were Alexa Fluor 555-labeled anti-rabbit antibodies (Molecular Probes, Eugene, OR, USA) and Alexa Fluor 488-labeled anti-mouse antibodies (Molecular Probes). Both.

Cells were incubated for 45 min at 37 °C with 4,6-diamidino-2-phenylindole dihydrochloride (Molecular Probes, Invitrogen, Cergy Pontoise, France), Alexa Fluor 488 Phalloidin (Molecular Probes,

The following protocol covers the 5 major steps of smFISH: A. Probe design and synthesis, B. Fixation of C. elegans worms and embryos, C. Hybridization, D. Image acquisition, E. Data analysis. This protocol is largely adapted from the general smFISH protocol detailed in Raj and Tyagi (2010), with notes and modifications specific to C. elegans.Unless otherwise noted, all reagents listed can be.

or anti-mouse Alexa 488 (green) antibodies at 1/4000 (Molecular Probes). Confocal imaging was performed using a Zeiss LSM 5 Pa confocal microscope. Freshly isolated reticulocytes from PHZ-treated or.

Alexa 488 anti-mouse, Alexa 568 anti-mouse, Alexa 568 anti-rabbit and Alexa 647 anti-rat (1:400, A11034, A11029, A11031, A11011, A21247, Molecular Probes). Samples were mounted with Vectashield.

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BD BiosciencesFluorochrome Reference Chart W D Stain Index = D/W Resolution sensitivity (the ability to resolve a dim positive signal from background) is a function of the difference between positive and background peak means (D) and the spread of the

Flow cytometry-based analysis. Jurkat cells were mock-induced with 0.2% DMSO or induced with 5 µM MG-132 overnight at 37°C. After treatment, cells were fixed and incubated with PROTEOSTAT® dye, then analyzed by flow cytometry without washing using a 488 nm laser in the FL3 channel.

IIIIIIII Wavelength (nm) 350 400 450 500 550 600 650 700 For more information on Bio-Rad imaging products, contact your local Bio-Rad representative.

Secondary antibodies used were Alexa-Fluor-488-labelled anti-mouse (Molecular Probes A11029; 1:400) and Alexa-Fluor-647-labelled anti-rat (Molecular Probes A21247; 1:400). DNA was stained with 5 mg ml.

followed by incubation for 1 h at room temperature with Alexa-Fluor-488- or Alexa-Fluor-594-labeled secondary antibodies (1:1000; Molecular Probes, Oregon, USA). The nuclei were stained with Hoechst.

After three washes with PBS, the upper chamber portions of the slides were removed, and the fixed cells were mounted and preserved with Prolong Anti-Fade (Molecular Probes). Negative controls for.

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Primarily antibodies used were monoclonal antibodies against active Caspase 3 (Promega), Ki67 (BD Bioscience), HuC/D (Molecular Probes), Chx10 (Exalha. Sections were then treated with Alexa-488-,

Bill Nyes Science Credentials Bill Nye frolicked in a ball pit to explain how the planet’s populations compete for resources. He took a chain saw to a loaf. President Donald Trump has taken a swipe at Joe Biden over the Democrat’s involvement with the 1994 Crime Bill, touting his. Science commentator Bill Nye had some choice words about the

Early-phase pathologies of Alzheimer’s disease (AD) are attracting much attention after clinical trials of drugs designed to remove beta-amyloid (Aβ) aggregates failed to recover memory and.

Jan 08, 2014  · To test the hypothesis that mode of binding to the TfR would affect transcytosis, we engineered two different types of Brain Shuttle constructs with a single-chain (sc) Fab fragment of an anti-TfR monoclonal antibody (mAb) fused either to one or both C-terminal ends of the heavy chain of an anti-Aβ mAb (mAb31).

Guide to Gold Cluster Labeling. If you want a detailed description of gold cluster labeling, isolating the conjugates and how to calculate the extent of labeling, then.

Membranes were incubated with anti-CRAF (BD Biosciences, 610152) or anti-BRAF (Sigma, WH0000673M1) primary antibodies, and analysed by the addition of a secondary Alexa Fluor 488 goat anti-mouse IgG.

Alexa Fluor 488 anti-mouse secondary antibody optimized for ICC/IF, IHC-Fr, Flow Cyt. Goat anti-mouse Alexa 488 secondary antibody binds to antibodies raised in mouse. See 24 publications. Find Alexa

Apparent molecular weight for several bands (asterisks. Secondary antibodies used were donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (A21206, Invitrogen), goat anti-rabbit IgG conjugated to.

Next day, sections were rinsed in PBST and incubated for 2 h in a cocktail of the corresponding secondary antibodies conjugated to either Alexa 488 or 555 fluorophores (Molecular Probes). To detect.

. with specific primary antibodies followed by Alexa-546-labeled goat anti-mouse antibody or Alexa-488-labeled goat anti-rabbit antibodies (Molecular Probes). DNA was stained using ToPro3 (Molecular.

The corresponding secondary antibodies were obtained from Molecular Probes Thermo Fisher and listed as follows: Alexa Fluor 350 anti-mouse IgG2b (A-21140), Alexa Fluor 488 anti-mouse IgG1 (A-21121).

Introduction. The dramatic increase in multi-color fluorescence microscopy applications witnessed over the past decade is due, in part, to the significant advances in instrument and detector design as well as the introduction of a vast array of new fluorophores, including synthetics and quantum dots.

Antigen-antibody complexes were visualized by goat anti-rabbit IgG H&L (Alexa Fluor 488) and goat anti-mouse IgG H&L (Alexa. Fluor 594–conjugated secondary antibodies (1:200; Molecular Probes/Life.

Antibody staining was revealed using species-specific fluorophore-conjugated secondary antibodies (Cy5 from Jackson, West Grove, PA, USA, Alexa 488 from Molecular Probes, San Diego, CA, USA). Cre.

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Alexa 488 IgG1, Alexa 594 IgM (A21140, A21121, A21044 respectively; Molecular Probes by Life Technologies) for one hour at RT. Slides underwent a final wash before being mounted. iWAT samples were.

Obesity, a growing health problem in western societies, is associated with increased senescent cells and neuropsychiatric disorders, including anxiety and depression. Ogrodnik and colleagues found that clearance of senescent cells in obese mice alleviates anxiety. Our study provides proof-of-concept evidence that senolytics are a potential new therapeutic avenue for treating neuropsychiatric.

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Introduction. Fluorescence is a member of the ubiquitous luminescence family of processes in which susceptible molecules emit light from electronically excited states created by either a physical (for example, absorption of light), mechanical (friction), or chemical mechanism. Generation of luminescence through excitation of a molecule by ultraviolet or visible light photons is a phenomenon.

Alexa Fluor 488 phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 495/518 nm Superior to antibody staining Optimal for fixe

PlantProbes is a distribution service for monoclonal antibodies and molecular probes directed to plant cell wall components generated by the Paul Knox Cell Wall Lab at the University of Leeds.Funds received are used to maintain antibody stocks and antibody availability.

Molecular hydrogen (H 2) was originally thought to behave as an inert gas in mammalian cells; however, our previous studies showed that this is not always the case 1, demonstrating that H 2.

The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and.

Figure 6: Schematic image showing the R-spondin 2 (Rspo2)-mediated acetylcholine receptor (AChR) clustering at the neuromuscular junction (NMJ. with the goat anti-rabbit Alexa 488 secondary.